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neural proliferation medium (neurocult™ proliferation kit  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc neural proliferation medium (neurocult™ proliferation kit
    Neural Proliferation Medium (Neurocult™ Proliferation Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/neural+proliferation+medium/us12024722-1096-23-26?v=STEMCELL+Technologies+Inc
    Average 90 stars, based on 1 article reviews
    neural proliferation medium (neurocult™ proliferation kit - by Bioz Stars, 2026-07
    90/100 stars

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    Effects of Passage on hDPSC Characteristics. (A) Cell <t>proliferation</t> capacity was assessed using MTT assays after 48 h. Proliferation decreased at passage 20 compared to passages 5 and 10. (B) Left panel: representative immunostained images of hDPSCs. (Blue: DAPI, Green: Phalloidin) Scale bar: 100 μm. Cell size increased with passage number. (C) Left panel: representative cell migration assay images of each time points were exhibited. Migration capacity decreased with passage number. (D) Expression of pluripotency and osteogenic differentiation markers (Sox2 and Bglap) decreased with passage number. Expression of osteogenic and adipogenic differentiation markers (ALP and LPL) also decreased at passage 20 compared to passages 5 and 10, while Nestin, neural differentiation marker remained unchanged. (E) osteogenic and adipogenic differentiation (Alizarin Red and Oil Red O staining respectively) decreased with passage number.
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    Effects of Passage on hDPSC Characteristics. (A) Cell <t>proliferation</t> capacity was assessed using MTT assays after 48 h. Proliferation decreased at passage 20 compared to passages 5 and 10. (B) Left panel: representative immunostained images of hDPSCs. (Blue: DAPI, Green: Phalloidin) Scale bar: 100 μm. Cell size increased with passage number. (C) Left panel: representative cell migration assay images of each time points were exhibited. Migration capacity decreased with passage number. (D) Expression of pluripotency and osteogenic differentiation markers (Sox2 and Bglap) decreased with passage number. Expression of osteogenic and adipogenic differentiation markers (ALP and LPL) also decreased at passage 20 compared to passages 5 and 10, while Nestin, neural differentiation marker remained unchanged. (E) osteogenic and adipogenic differentiation (Alizarin Red and Oil Red O staining respectively) decreased with passage number.
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    Effects of Passage on hDPSC Characteristics. (A) Cell proliferation capacity was assessed using MTT assays after 48 h. Proliferation decreased at passage 20 compared to passages 5 and 10. (B) Left panel: representative immunostained images of hDPSCs. (Blue: DAPI, Green: Phalloidin) Scale bar: 100 μm. Cell size increased with passage number. (C) Left panel: representative cell migration assay images of each time points were exhibited. Migration capacity decreased with passage number. (D) Expression of pluripotency and osteogenic differentiation markers (Sox2 and Bglap) decreased with passage number. Expression of osteogenic and adipogenic differentiation markers (ALP and LPL) also decreased at passage 20 compared to passages 5 and 10, while Nestin, neural differentiation marker remained unchanged. (E) osteogenic and adipogenic differentiation (Alizarin Red and Oil Red O staining respectively) decreased with passage number.

    Journal: Regenerative Therapy

    Article Title: Exploring SSEA3 as an emerging biomarker for assessing the regenerative potential of dental pulp-derived stem cells

    doi: 10.1016/j.reth.2024.05.004

    Figure Lengend Snippet: Effects of Passage on hDPSC Characteristics. (A) Cell proliferation capacity was assessed using MTT assays after 48 h. Proliferation decreased at passage 20 compared to passages 5 and 10. (B) Left panel: representative immunostained images of hDPSCs. (Blue: DAPI, Green: Phalloidin) Scale bar: 100 μm. Cell size increased with passage number. (C) Left panel: representative cell migration assay images of each time points were exhibited. Migration capacity decreased with passage number. (D) Expression of pluripotency and osteogenic differentiation markers (Sox2 and Bglap) decreased with passage number. Expression of osteogenic and adipogenic differentiation markers (ALP and LPL) also decreased at passage 20 compared to passages 5 and 10, while Nestin, neural differentiation marker remained unchanged. (E) osteogenic and adipogenic differentiation (Alizarin Red and Oil Red O staining respectively) decreased with passage number.

    Article Snippet: Briefly, for neuronal differentiation, hDPSCs were cultured in serum-free human neural proliferation medium (StemCell Technologies, Canada) supplemented with epidermal growth factor (20 ng/ml) and basic fibroblast growth factor (10 ng/ml) as growth factors and 1% antibiotics at 37 °C in a 5% CO 2 atmosphere.

    Techniques: Cell Migration Assay, Migration, Expressing, Marker, Staining